Identification of Additional Calmodulin Binding Regions in Connexin43
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چکیده
منابع مشابه
Identification and characterization of the calmodulin-binding domain of neuromodulin, a neurospecific calmodulin-binding protein.
Neuromodulin (formerly designated P-57) is an abundant, neural specific, calmodulin-binding protein which exhibits higher affinity for calmodulin in the absence of free Ca2+ than in the presence of free Ca2+. In this study a series of proteolytic fragments of neuromodulin were systematically screened for calmodulin-Sepharose binding activity. A 9-amino acid fragment, designated M1-C1 and having...
متن کاملIdentification of the Regions Conferring Calmodulin-like Properties to Troponin
The structural and functional correlations between troponin C (TnC) and calmodulin (CaM) were investigated by mutagenizing a synthetic cDNA coding rabbit skeletal muscle TnC. Compared with TnC, calmodulin lacks the N-terminal a-helical arm (N-helix), and its central helix is shorter due to the absence of SaKGKso residues. Deleting both regions concomitantly (ANtAKGK) elicited CaM-like regulatio...
متن کاملIdentification of Calmodulin-binding Proteins in Chicken Embryo Fibroblasts
We recently reported the detection of multiple classes of calmodulin-binding proteins in subcellular fractions of chicken embryo fibroblasts by using a gel binding procedure (Van Eldik, L . J ., and W. H . Burgess, 1983, J . Biol. Chem ., 258:4539-4547) . In this report we identify many of these calmodulin-binding proteins and provide further evidence for the existence of multiple classes of ca...
متن کاملIdentification of calmodulin-binding proteins in chicken embryo fibroblasts
We recently reported the detection of multiple classes of calmodulin-binding proteins in subcellular fractions of chicken embryo fibroblasts by using a gel binding procedure (Van Eldik, L.J., and W.H. Burgess, 1983, J. Biol. Chem., 258:4539-4547). In this report we identify many of these calmodulin-binding proteins and provide further evidence for the existence of multiple classes of calmodulin...
متن کاملIdentification of the calmodulin binding domain of connexin 43.
Calmodulin (CaM) has been implicated in mediating the Ca(2+)-dependent regulation of gap junctions. This report identifies a CaM-binding motif comprising residues 136-158 in the intracellular loop of Cx43. A 23-mer peptide encompassing this CaM-binding motif was shown to bind Ca(2+)-CaM with 1:1 stoichiometry by using various biophysical approaches, including surface plasmon resonance, circular...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.607